Recombinant Human TOPK (N-GST tag) | Z500391

abm | Recombinant Human TOPK (N-GST tag) | Z500391

TOPK is a MAPK kinase that phosphorylates p38 MAPK and is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro . Expression of TOPK is detected in male germ line progenitor cells, activated T-cells, and a variety of lymphomas and leukemias. In vitro studies have shown that activated TOPK phosphorylated p38MAPK but not JNK or ERK. TOPK activation requires phosphorylation by both the M-phase CDK1/CyclinB kinase complex and another unknown kinase, possibly RafC or RafA. TOPK may play an important role in linking extracellular signals to an intracellular state, possibly allowing extracellular influence on the cell-cycle-related processes of proliferation or differentiation .

Functions:

The specific activity of TOPK was determined to be 7.5 nmol /min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

PBK, SPK, Nori-3, FLJ14385

Gene Symbol:

TOPK

Gene ID:

N/A

Accession:

NM_018492

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

68

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.