Recombinant Human ULK1 (N-GST tag) | Z500402

abm | Recombinant Human ULK1 (N-GST tag) | Z500402

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR . ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy . Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

Functions:

The specific activity of ULK1 was determined to be50nmol /min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

ATG1, UNC51, Unc51.1, FLJ38455

Gene Symbol:

ULK1

Gene ID:

N/A

Accession:

BC111603

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

125

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.