Recombinant Mouse SHH | Z200619

abm | Recombinant Mouse SHH | Z200619

Mouse SHH is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. Mouse SHH shares 99% homology with the human gene for SHH. Sonic HedgeHog is a protein that is vital in guiding the early embryo; it has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. SHH binds to the patched receptor to activate the transcription of target genes. Sonic HedgeHog also regulates another factor, the gli oncogene. Recombinant Mouse SHH is a single, non-glycosylated polypeptide chain of 19.8 kDa.

Functions:

The ED50 of this protein, as measured by its ability to induce alkaline phosphatase production in mouse myoblast cell, was in the range of 500ng-3 ug/mL.

Organism:

Mouse

Source:

E. coli

Alias:

Sonic hedgehog protein, HHG-1,

Gene Symbol:

SHH

Gene ID:

20423

Accession:

Q62226

Formulation:

Lyophilized from a 0.2 µm filtered solution in phosphate buffer and NaCl

Solubility:

N/A

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

19,8

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.