RNaseOFF Ribonuclease Inhibitor | G138
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abm | RNaseOFF Ribonuclease Inhibitor | G138
RNaseOFF Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation over the highly oxidation-sensitive human RNase inhibitor. RNaseOFF Ribonuclease Inhibitor is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.
One unit is defined as the amount of RNaseOFF Ribonuclease Inhibitor that is required to inhibit the activity of 5 ng of RNase A by 50%.
• Always keep RNaseOFF on ice during reaction set-up.
• It is recommended to setup all RNA-related/RNase-sensitive work under conditions where RNase contamination has been eliminated. Pipette tips and tubes should be treated with 0.1% DEPC.
• Wearing gloves is highly recommended while working with RNA.
This product is distributed for laboratory research only Caution: Not for diagnostic use.
The source of this protein is a recombinant E. coli strain encoding pyrophosphatase from Thermococcus litoralis.
Store all components at -20°C.
4000 U (100 µl)