SEP-PTHR Stable Expressing Cell Line | T6301

abm | SEP-PTHR Stable Expressing Cell Line | T6301

SEP-PTHR Stable Expressing Cell Line were established via stable transfection of HEK293 cells with the Supereclipyic pHluorin (SEP)-tagged PTHR plasmid (pH-sensitive GFP variant). The stable cells confer resistance to G418 in culture, and has the ability to respond to parathyroid hormone stimulation.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 48 hours

Seeding Density:

N/A

Markers:

G418 resistant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Note: selection antibiotics should not be added upon thawing or for routine cell propagation. Let cells recover first after thawing, then once they grow well put for 7 days in selection medium. Then grow again in normal medium.
The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C