Site-Directed Mutagenesis Kit | E088

abm | Site-Directed Mutagenesis Kit | E088

abm’s Site-Directed Mutagenesis Kit allows for efficient and rapid mutagenesis of double-stranded plasmid DNA. It enables the generation of point mutations, deletions and insertions at any site without design constraints. Site-directed mutagenesis is a useful tool to study changes in gene or protein function as well as easily make vector modifications. ProClone ™ Competent Cells are recommended for use with this kit for the highest quality results.
Advantages:

• Use of standard primers eliminates additional primer expenses – no phosphorylation step is required.
• Ready-to-use 2X Bestaq™ MasterMix with high-fidelity DNA polymerase limits unwanted PCR-introduced errors.
• PCR treatment reagents rapidly eliminate the template background without lengthy hands-on purification steps.
• Pro Ligation-Free MasterMix results in highly efficient, seamless assembly of the mutant plasmid
• Condensed 4 hour workflow, with less than 15 minutes of hands-on time for highly efficient and accurate mutagenesis.

Concentration:

N/A

Form:

N/A

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

N/A

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

MfeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer

Inactivation:

No

Source:

An E.coli strain that carries the modified MfeI gene from Mycoplasma fermentas (N.F. Halden). The MfeI enzyme recognizes and cuts the sequence C^AATTG.

Shipping conditions:

Ice Packs

Storage conditions:

N/A

Storag Buffer:

N/A