Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line | T6080

abm | Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line | T6080

The Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line was generated from embryos of a pregnant mouse heterozygous for the bcl-x gene (bcl-x^+/−). This stable cell line lacks the ability to express B-cell lymphoma-extra-large (Bcl-x_L) but this deficiency did not induce compensatory changes in the expression of other Bcl-2 proteins. The Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line showed increased sensitivity to various apoptotic stimuli. This cell line is also capable of re-expressing wild-type and Bcl-x_L mutants which makes it a useful research tool in demonstrating how apoptotic regulation is involved in disease development.

Biosafety:

II

Organism:

Mouse

Source Organ:

Embryo

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

35 - 45 hours

Seeding Density:

30,000 - 40,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Western blot analysis of Bcl-xL expression; 2) Microarray analysis

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C