Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line | T6084

abm | Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line | T6084

The Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line was derived from embryos of a pregnant mouse heterozygous for the bcl-x gene (bcl-x+/−). After immortalization by transfection of the SV40 large T antigen, the mouse embryonic fibroblasts were transfected with mutant Bcl-x_L cDNA. This stable cell line is able to express B-cell lymphoma-extra-large (Bcl-x_L) localized in the endoplasmic reticulum (ER). Bcl-x_L is a major regulator of cellular apoptotic signalling by expressing Bcl-2 proteins. The Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line is a useful model in studying apoptosis regulation of tissue differentiation and homeostasis focusing on the specific activities of Bcl-x_L at the endoplasmic reticulum as well as how Bcl-x_L functions in the regulation of Ca²⁺ signalling. This cell line is also useful in screening and development of new therapeutic strategies for cancer and other diseases.

Biosafety:

II

Organism:

Mouse

Source Organ:

Embryo

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfected with mutant Bcl-x_L cDNA

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Western blot analysis of Bcl-xL expression; 2) Microarray analysis

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C