Stable HEK293 Expressing Rat P2X7 Cell Line (HEK rt p2x7) | T6315

abm | Stable HEK293 Expressing Rat P2X7 Cell Line (HEK rt p2x7) | T6315

Stable HEK293 Expressing Rat P2X7 Cell Line (HEK rt p2x7) were established through stable lipofectamine transfection of the rat PX27 receptor gene to parental HEK293 cells. Monoclonal selection via 1 mg/ml G418 was conducted to maintain a selection pressure. The cell line established may be used to study PX27 receptors and downstream pathways related to the receptor.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

48 hours

Seeding Density:

4,000 cells/cm²

Markers:

PX27, Neomycin resistance (0.3 mg/ml G418)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Expression of P2X7 receptor detection by Western blot using anti-P2X7 receptor antibody (Alomone, #APR-004)

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C