Stable HEK293T Expressing Human GC-B (GCB-Full) Cell Line | T6036

abm | Stable HEK293T Expressing Human GC-B (GCB-Full) Cell Line | T6036

The Stable HEK293T Expressing Human GC-B Cell Line (GCB-Full) contains the gene encoding for the full length guanylyl cyclase-B receptor (GC-B). The expression of the human GC-B was done by transfecting HEK293T cells using a bicistronic mammalian expression vector containing the GC-B. GC-B is related to conditions such as achondroplasia which makes the Stable HEK293T Expressing Human GC-B Cell Line (GCB-Full) useful in pharmacology for testing treatments for achondroplasia and human dwarfism.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Via transfection of bicistronic mammalian expression vector, pIRES2–AcGFP1

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Western blot of a membrane preparation (60 lg of protein); 2) FACS-based selection based on fluorescence intensity from AcGFP1; 3) Selection with 0.2 µg/ml puromycin

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C