Stable HEK293T Expressing Mouse DMT1 Cell Line | T6000

abm | Stable HEK293T Expressing Mouse DMT1 Cell Line | T6000

Stable HEK293T Expressing Mouse DMT1 Cell Line overexpresses the divalent metal transporter-1 (DMT1) introduced to the HEK293T cells via transfection with DMT1 cDNA. The cell line is useful for the cell-based screening of small molecule inhibitors of iron uptake due to metal-sensitive calcein fluorescence. Hence a unique feature is that in the presence of iron, calcien fluorescence is quenched. Therefore, the Stable HEK293T Expressing Mouse DMT1 Cell Line may be used for calcein fluorescence quenching to assay for iron transporter inhibitors to gain more insight on iron uptake.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Divalent metal transporter-1 (DMT1) via transfection

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII medium available at abm

Preservation:

N/A

Quality Control:

1) puromycin resistance at 2 µg/ml, 2) Western blot used to determine presence of DMT1

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C