Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line | T3195
- 5 to 7 Days Shipment
abm | Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line | T3195
Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line is recommended for use to study the production of acetaldehyde and its effects in dysfunctions in alcoholic liver disease. The cell line expresses mouse ADH1 (Class 1 Alcohol Dehydrogenase) which oxidizes allyl alcohol to produce toxic acetaldehyde. The Va-13 cells may use used to investigate ethanol metabolism.
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
For Research Use Only
Transfection with pIVL-2 vector encoding for mouse ADH1 (Class 1 Alcohol Dehydrogenase)
The base medium for this cell line isPrigrow III medium available at abm
1) 400 μg/mL zeocin for selection; 2) Redox state was measured by levels of lactate and pyruvate released in culture medium.
10⁶ cells/1.0 ml