Stable WM115 Expressing RAGE Cell Line | T6013

abm | Stable WM115 Expressing RAGE Cell Line | T6013

Stably Overexpressing Thymidine Kinase Human Mesenchymal Stem Cell Line is applicable for studies for cancer gene therapy. It has capabilities of self-renewal and tumor-homing ability and with low immunogencity can become a potential mode of suicide gene delivery. It may be used to study the biological functions of the thymidine kinase.

Biosafety:

II

Organism:

Human

Source Organ:

Skin

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfected with pcDNA3 expressing full-length RAGE (receptor for advanced glycation end products)

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Opti-MEM Invitrogen). To make the complete growth medium, add the following components to the base medium: fetal bovine serum

Preservation:

N/A

Quality Control:

1) 1 mg/ml G418 for selection; 2) RAGE was detected via RT-PCR and ELISA; 3) Western blot used to analyze S100B and p53 levels

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C