Description
abm | SwaI | E061
N/A
Concentration:
20000 U/ml
Form:
N/A
Alternate Name:
N/A
Reaction Buffer:
Buffer 1: 10%, Buffer 2: 10%, Buffer 3: 100%, Universal Buffer: 10% Click here to view the buffer compatibility chart for restriction enzymes.
Reaction:
1X Restriction Enzyme Reaction Buffer 3 (Cat.No. E203) Incubate at 25°C. SwaI is unstable at 37°C and has reduced activity if incubated at 37°C.
Notes:
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive
Application:
Restriction enzyme digestion of double-stranded DNA
Components:
NcoI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Inactivation:
80°C for 20 min
Source:
An E. coli strain that carries the modified NcoI gene from Nocardia carolina (ATCC 19070). The NcoI enzyme recognizes and cuts the sequence C^CATGG.
Shipping conditions:
Ice Packs
Storage conditions:
-20°C
Storag Buffer:
10mM Tris pH (7.5), 400mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol.
Discount(%):
30