T4 Gene 32 Protein | E040

abm | T4 Gene 32 Protein | E040

T4 Gene 32 Protein is a single-stranded DNA (ssDNA) binding protein which is required for T4 bacteriophage replication, recombination and repair. T4 Gene 32 Protein binds cooperatively and stoichiometrically to ssDNA effectively stabilizing it. This protein enhances DNA synthesis activities, including PCR amplification and DNA sequencing.

Concentration:

10 mg/ml

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

This product is distributed for laboratory research only Caution: Not for diagnostic use.

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

NotI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer

Inactivation:

65°C for 20 min

Source:

An E. coli strain that carries the modified NotI gene from Nocardia otitidis-caviarum (ATCC 14630). The NotI enzyme recognizes and cuts the sequence GC^GGCCGC.

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

10 mM Tris-HCl (pH 7.5), 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.