3xFLAG-AGO2 Stable Expressing HEK293T Cell Line | T6352

abm | 3xFLAG-AGO2 Stable Expressing HEK293T Cell Line | T6352

MicroRNAs (miRNAs) comprise a class of small non-coding RNAs that regulate the stability and/or translatability of most protein-coding transcripts. They guide AGO proteins within the RNA-induced silencing complex (RISC) and plays a part to degrade or repress the translation of their target mRNAs. 3xFLAG-AGO2 stable expressing HEK293T cells can allow you to investigate the susceptibility of AGO2-loaded miRNA to different classes of nucleases and can be used in complement to in vivo studies to identify new miRNA decay factors.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

Express AGO2, Express FLAG-AGO2

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 5% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) ANTI-FLAG®M2 Affinity Gel immunoprecipitation

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C