AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line | T3104

abm | AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line | T3104

The androgen receptor (AR) is a ligand-activated transcription factor involved in developmental and psychological processes and implicated in the pathogenesis of prostatic hyperplasia, prostate cancer, and altered pubertal development. The AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line contains a reporter plasmid with three copies of the androgen response region with a downstream androgen response element which allows for the detection of AR ligands through the induction of luciferase activity as early as 8 hours after treatment. The reporter plasmid p3ARR/ARE-luc2P/minP/hygro was successfully transfected into the 22Rv1 cells using the Fugene HD transfection reagent. These immortalized cells are functional for over 25 passages after cryopreservation making them a reproducible and sensitive tool for the detection of AR ligands during research investigating novel pharmaceutical drugs or screening for environmental pollutants that may disrupt endocrine function through AR interaction.

Biosafety:

II

Organism:

Human

Source Organ:

Prostate

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

49 - 59 hours

Seeding Density:

40,000 cells/cm² to 80,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

Selective growth on media containing hygromycin B

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C