PZ-TR Stable HepG2 Luciferase Reporter Cell Line | T3177

abm | PZ-TR Stable HepG2 Luciferase Reporter Cell Line | T3177

PZ-TR Stable HepG2 Luciferase Reporter Cell Line is designed to assess the transcriptional activity of the human thyroid receptor (TR) through a luciferase reporter system. The presence of thyroid hormones, such as the triiodothyronine (T3), would induce luciferase activity and allows for function assessment. It is a valuable tool for the field of endocrine disruptor research as the cell line preserves the stoichiometric ratio between the human TR receptor and other transcriptional regulators.

Biosafety:

II

Organism:

Human

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Human thyroid receptor (TR) via transfection of transcriptionally active TR pGL4.14-TRE plasmid and selected by 500μg/mL hygromycin B for clonal selection

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Resistance to 500μg/mL hygromycin B for clonal selection; 2) Luciferase activity with 10 nM T3 after 48 hour incubation.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C