CA-MEK5 Stably Expressing HCT116 Cell Line | T6444
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abm | CA-MEK5 Stably Expressing HCT116 Cell Line | T6444
HCT116 cells were transduced with lentiviral particles carrying pWPI-eGFP expression constructs encoding CA-MEK5 (MEK5DD), followed by fluorescence-activated cell sorting to generate CA-MEK5 Stably Expressing HCT116 Cell Line. Mitogen/extracellular signal-regulated kinase kinase-5, MEK5, has been associated with tumor carcinogenesis, such as aberrant MEK5–ERK5 pathway signaling have been reported in cancer metastasis. CA-MEK5 Stably Expressing HCT116 Cell Line may be a useful tool to study cancer biology and it's role in cancer invasion and metastasis.
Human (H. sapiens)
For Research Use Only
Use of PriCoatTM T25 Flasks G299) or Applied Cell Extracellular Matrix G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
1) Cell sorted for GFP expression; 2) Immunoblotting perfromed for MEK5 expression.
10⁶ cells/1.0 ml