Conditionally Immortalized Stria Vascularis Cell Line (SV-k1) | T0784
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abm | Conditionally Immortalized Stria Vascularis Cell Line (SV-k1) | T0784
Derived from transgenic mouse which constitutively expresses a temperature-sensitive simian virus 40 T antigen (tsA58).
Prestin (-), occulin (+)
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 10%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
To differentiate cells: after growing cells to 80% confluence at 33.0°C, move cells to 39.0°C and 5% CO2 conditions for 2-3 weeks. Cells will slowly stop growing and will die off, with all cells dying after approximately 4 weeks.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml