Exonuclease III, E. coli | E016

abm | Exonuclease III, E. coli | E016

Exonuclease III, E.coli digests duplex DNA in the 3´→5´ direction from nicked DNA, blunt end DNA, 3´-recessed ends, or 3´-overhangs of less than four bases, and yields nucleoside 5´-phosphates. The DNA degradation proceeds at a uniform rate and produces stretches of ssDNA on the opposite strand. Under defined reaction conditions, the reaction can yield predictable and reproducible digestion results. Conditions such as temperature, ionic strength, template DNA sequence and the Exonuclease III to DNA ratio need to be optimized to suit specific applications to achieve the desired excision rate. Exonuclease III, E.coli is also capable of degrading DNA from 3´-phosphate ends due to intrinsic 3´-phosphatase activity. The enzyme also has apurinic DNA endonuclease activity as well as RNase H activity.

Concentration:

100 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

ApaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

Inactivation:

65°C for 20 min

Source:

An E. coli strain that carries the ApaI gene from Acetobacter pasteurianus sub. pasteurianus (ATCC 9432). The ApaI enzyme recognizes and cuts the sequence GGGCC^C.

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

20 mM Tris-HCl (pH 7.5), 100 mM KCl, 1 mM DTT, and 50% (v/v) Glycerol.