Exonuclease I, E. coli | E015

abm | Exonuclease I, E. coli | E015

Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3’-terminus is phosphorylated.

Concentration:

20 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

Sold by mass of pure protein as determined by OD₂₈₀.

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

AgeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

Inactivation:

65°C for 20 min

Source:

An E. coli strain that carries the cloned and modified AgeI gene from Ruegeria gelatinovora (ATCC 25655). The AgeI enzyme recognizes and cuts the sequence A^CCGGT.

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

20 mM HEPES (pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% (v/v) Glycerol.