Exonuclease I, E. coli | E015
- 5 to 7 Days Shipment
abm | Exonuclease I, E. coli | E015
Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3’-terminus is phosphorylated.
Enzyme supplied with 10X Reaction Buffer
Sold by mass of pure protein as determined by OD280.
Restriction enzyme digestion of double-stranded DNA
AgeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
65°C for 20 min
An E. coli strain that carries the cloned and modified AgeI gene from Ruegeria gelatinovora (ATCC 25655). The AgeI enzyme recognizes and cuts the sequence A^CCGGT.
Store all components at -20°C.
20 mM HEPES (pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% (v/v) Glycerol.
2000 U (100 μl)