T5 Exonuclease | E089

abm | T5 Exonuclease | E089

T5 exonuclease catalyzes the degradation of nucleotides either from the 5’ termini or at nicks of linear or circular dsDNA in a 5’ to 3’ direction. This exonuclease also exhibits ssDNA endonuclease activity in the presence of magnesium ions, but will not degrade supercoiled dsDNA.

Concentration:

10000 U/ml

Form:

Enzyme supplied with a 10X Reaction Buffer. Incubate at 37°C.

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of enzyme necessary to catalyze the release of 1 nmol of acid-soluble nucleotides from double-stranded DNA in a 50 µl volume in 30 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Application:

1. Rapid multi-fragment cloning and assembly of whole vector constructs.
2. Clone in a promoter, gene, and tag, all in one step.
3. Rapid assembly of CRISPR multiplex gRNA vectors in one step.

Components:

N/A

Inactivation:

N/A

Source:

N/A

Shipping conditions:

Ice Packs

Storage conditions:

Store all components at -20°C.

Storag Buffer:

50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol and 0.1% Triton X-100.