Immortalized Calf Parathyroid Cells-SV40 | T0411
- 5 to 7 Days Shipment
abm | Immortalized Calf Parathyroid Cells-SV40 | T0411
SV40 Large T Antigen
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. This cell line needs to be cultured in fibronectin coated flasks Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml