Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7 | T0352

abm | Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7 | T0352

Immortalization Method:

N/A

BioSafety Level:

II

Organism:

Canine

Species:

Dog

Source Organ:

Adipose Tissue

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

50-60 hours

Seeding Density:

N/A

Markers:

Puromycin

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, and 0.2 ?g/ml puromycin (G264).
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1. Real Time PCR was used to quantify HPV E6 and E7 gene expression in immortalized cell line. 2. Puromycin selection

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C