Immortalized Human Hepatocytes - HPV E6/E7, hTERT & MycT58A | T0063
- 5 to 7 Days Shipment
abm | Immortalized Human Hepatocytes - HPV E6/E7, hTERT & MycT58A | T0063
Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7, hTERT & MycT58A genes
30 - 35 hours
5 - 15,000 cells/cm10²; Recommended split ratio of 1:2 to 1:5*
* Do not exceed a split ratio of 1:5
Grow cells in culture vessels coated with Collagen I, rat tail. Coat plates at 37.0°C overnight and wash with sterile PBS prior to use.
The base media for this cell line is Prigrow IX medium available at abm Cat. No. to a final concentration of 10%, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml