Immortalized Drosophila Embryonic Cells (R4) | T0703
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abm | Immortalized Drosophila Embryonic Cells (R4) | T0703
Serial passaging of primary cultures from Act5C > UAS-RasV12 embryos
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for this cell line is Schneider's Insect Medium (TM034). To make the completed growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%, and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 22.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
(1) Inducing muscle cell differentiation by treating cells with ecdysone and perform immunofluorescence staining and qPCR to assess the upregulated expression of mhc and Tropomyosin (Tm).
(2) Up-regulated genes in DPCs are analyzed by DAVID (the Database for Annotation, Visualization and Integrated Discovery).
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml