Immortalized Human Adult Brain Astrocytes (AST-1) | T0783

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SKU:
T0783
Availability:
5 to 7 Days Shipment
€750.00
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Description

abm | Immortalized Human Adult Brain Astrocytes (AST-1) | T0783

Immortalization Method:

Immortalized via introduction of cytoplasmic content (non-DNA) of tumor cells into primary cells

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

24 - 30 hours

Seeding Density:

20,000 - 40,000 cells/cm2

Markers:

GFAP

Donor Age:

Not disclosed

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

Use of PriCoatTM T25 Flasks or Applied Cell Extracellular Matrix is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow IV medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%, Penicillin/Streptomycin Solution to a final concentration of 1%, Recombinant Human FGF2 to a final concentration of 10 ng/ml, Recombinant Human GH1 to a final concentration of 10 ng/ml, and Recombinant Human HGF to a final concentration of 10 ng/ml.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) IEF; 2) IHS

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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Additional Information

Size:
1x10⁶ cells / 1.0 ml
Quantity:
Inquire
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