Immortalized Human Amniotic Epithelial Cells - SV40 | T0531
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abm | Immortalized Human Amniotic Epithelial Cells - SV40 | T0531
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml