Immortalized Human Ovarian Epithelial Cells - SV40 | T1074
- 5 to 7 Days Shipment
abm | Immortalized Human Ovarian Epithelial Cells - SV40 | T1074
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
19 - 29 hours
15,000 - 30,000 cells/cm10²; Recommended split ratio is 1:3 to 1:4
The base medium for this cell line is Prigrow I medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml