Immortalized Marmoset Hepatic Progenitor Cells | T0719

abm | Immortalized Marmoset Hepatic Progenitor Cells | T0719

Immortalization Method:

Immortalized with lentivirus containing the SV40 T antigen

BioSafety Level:

II

Organism:

Marmoset

Species:

Marmoset

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent

Morphology:

Elongated spindle

Passage Number:

N/A

Population Doubling:

55 hours

Seeding Density:

N/A

Markers:

Hepatic progenitor markers (CD44, Sox9, Sox17 and Ncam1)

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base culture medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, 2-mercaptoethanol to a final concentration of 0.1 mM, 10 ng/ml hepatocyte growth factor (HGF) to a final concentration of 10 ng/ml, Recombinant Human EGF to a final concentration of 10 ng/ml, insulin-transferrin-selenium (Invitrogen) to a final concentration of 1X, dexamethasone (Sigma) to a final concentration of 10^-7 M, and nicotinamide (Sigma) to a final concentration of 10 ng/ml.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Karyotype and telomere analysis; 2) RNA sequencing; 3) Reverse transcription-PCR; 4) Immunocytochemistry and immunohistochemistry; 5) Histology; 6) PAS, ICG, and CDFDA uptake assays and Oil Red O staining; 7) In vitro differentiation; 8) CYP450 induction

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C