Immortalized Mouse Cardiomyogenic Progenitor Cells (iCP) | T0449
- 5 to 7 Days Shipment
abm | Immortalized Mouse Cardiomyogenic Progenitor Cells (iCP) | T0449
Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites
CD1 Mouse Embryo
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
1) RT-PCR, qPCR, and sqPCR to detect cell markers; 2) Western blot analysis for SV40 large T antigen presence.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml