Immortalized Mouse Embryonic Hepatic Progenitor Cells (iHP) | T0129
- 5 to 7 Days Shipment
abm | Immortalized Mouse Embryonic Hepatic Progenitor Cells (iHP) | T0129
Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites
CD1 Mouse Embryo
30 - 40 hours
10,000 - 30,000 cells/cm10²
CD34, Pou4f1/Oct4, AFP, Dlk, Alb
The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1% Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
1) qPCR to examine expression of cell markers; 2) Western blot analysis for SV40 large T antigen; 3) Indocyanine green (ICG) uptake and release assay and periodic acid-schiff (PAS) staining were performed to determine cell functions.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml