Immortalized Mouse Hepatocyte SIMH (CLOCK) Cells | T0750

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T0750
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5 to 7 Days Shipment
€4,173.00
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Description

abm | Immortalized Mouse Hepatocyte SIMH (CLOCK) Cells | T0750

CLOCK Cells contain a bioluminescent marker gene that reports on the circadian phase of the cells. The presence of the Per2:Luc reporter gene allows real time monitoring of clock phase. The major characteristics of this cell line include its high metabolic capacity, liver-like properties, robust synchronizable circadian clock, and capacity to stably express additional marker. When compared with other established cell lines (i.e. HepG2), the SIMHCLOCK cells show a significantly better metabolic and xenobiotic capacity. This ability of the Immortalized Mouse Hepatocyte SIMHCLOCK Cells is useful in studying treatment of liver cells with various physical or chemical compounds at defined stages of the circadian cycle and analyze the time-of-day exposure effects.

Immortalization Method:

Transfection with mammalian expression vector carrying SV40 gene

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IX medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Bioluminescence recording of luciferase activity; 2) Apoptosis assay using exposure to B[a]P; 3) qPCR; 4) RNA Seq analysis

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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Additional Information

Size:
1x10⁶ cells / 1.0 ml
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