Immortalized Human Adult Hepatocyte Cells (MHep-2) | T0779

abm | Immortalized Human Adult Hepatocyte Cells (MHep-2) | T0779

Immortalization Method:

Immortalized via introduction of cytoplasmic content (non-DNA) of tumor cells into primary cells

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Suspension

Morphology:

Round

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Phenylalanine hydroxylase, alpha-1 antitrypsin, Albumin, CYP3A4

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 5%, 10 ng/ml human bFGF, 10 ng/ml human HGF and 10 ng/ml human Growth Hormone, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) IHC

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C