Immortalized Mouse Myeloid-derived Suppressor-like (HD1A) Cells | T0137

abm | Immortalized Mouse Myeloid-derived Suppressor-like (HD1A) Cells | T0137

abm, T3187), in which lysopmal acid lipase (LAL) expression is observed. Together HD1A and HD1B cells may be used as an in vitro system for studies relating to LAL and myeloid functions.

Immortalization Method:

Immortomouse expressing a temperature-sensitive version of simian virus 40 large T antigen

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Peritoneal

Organ Type:

Blood

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available in abm. to final concentration of 10%, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C