Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line | T3187

abm | Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line | T3187

Peritoneal macrophages were collected from lal-/- male mice mice that had been crossbred with Immortomouse expressing a temperature-sensitive version of simian virus 40 large T antigen to generate the Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line. Myeloid-derived suppressor cells are myeloid progenitors that are blocked to further differentiate and may have a role in tumor proliferation. The cells lack the lysopmal acid lipase (LAL) expression in which the wild type is available for comparison, the Immortalized Mouse Myeloid-derived Suppressor-like (HD1A) Cells (abm, T0137). Together HD1A and HD1B cells may be used as an in vitro system for studies relating to LAL and myeloid functions.

Biosafety:

II

Organism:

Mouse

Source Organ:

Peritoneal

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

Cells were derived from lal-/- male mice crossed with immortomouse

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

1. Pre-warm complete media in a 37°C waterbath.
2. Remove the cryopreserved vial from the liquid nitrogen storage tank.
3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells.
4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media.
5. Centrifuge cells at 200x g for 3 minutes to pellet.
6. Aspirate out the media, leaving cell pellet undisturbed.
7. Re-suspend pellet in fresh culture medium and plate in new culture vessel.
8. Incubate cultures at 33°C, 5% CO₂.

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C