Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC-GFP) | T0543
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abm | Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC-GFP) | T0543
abm also offers the Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC) (abm, T0542) which does not carry GFP expression.
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for the cell line is Claycomb medium (Sigma-Aldrich). To make the completed growth medium, add the following components to the base medium: 2mM L-glutamine , fetal bovine serum to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
1) RT-PCR and Cytofluorimetry were performed on the cDNA from the cells to confirm the expression of stem cell markers Sca-1, c-kit, Islet 1, nestin, and nucleostemin.
2) Cells after differentiation were specifically stained with alizarin red, adipo-red and Alcian blue, and the tissue-specific collagen II antibodies, respectively to confirm osteogenic, adipogenic, and chondrogenic phenotypes the cells’ multipotency.
3) G-banding analysis for macroscopic chromosome alterations over time compared with normal mouse karyotypes.
4) GFP transduction was confirmed by culturing with antibiotics.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml