Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC) | T0542

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T0542
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Description

abm | Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC) | T0542


It displays a normal karyotype and stable telomeres, neither proliferates when plated in soft agar nor gives rise to tumors when injected subcutaneously in NOD/SCID-g null mice. The cell line is thus recommended as a valuable tool for a number of applications in ex vivo tissue engineering to study the differentiation mechanisms involved in tissue repair, as well as a model for both pharmacological and toxicological studies.

Immortalization Method:

Spontaneous immortalization

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Adipose tissue

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for the cell line is Claycomb medium (Sigma-Aldrich). To make the completed growth medium, add the following components to the base medium: 2mM L-glutamine , fetal bovine serum ro a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) RT-PCR and Cytofluorimetry were performed on the cDNA from the cells to confirm the expression of stem cell markers Sca-1, c-kit, Islet 1, nestin, and nucleostemin.

2) Cells after differentiation were specifically stained with alizarin red, adipo-red and Alcian blue, and the tissue-specific collagen II antibodies, respectively to confirm osteogenic, adipogenic, and chondrogenic phenotypes the cells’ multipotency.

3) G-banding analysis for macroscopic chromosome alterations over time compared with normal mouse karyotypes.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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Additional Information

Size:
1x10⁶ cells / 1.0 ml
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