abm immortalized cells
Immortalized Rat Ovarian Granulosa Cells (RGA-1) | T0758
- SKU:
- T0758
- Availability:
- 5 to 7 Days Shipment
Description
abm | Immortalized Rat Ovarian Granulosa Cells (RGA-1) | T0758
Immortalization Method:
Immortalized with a temperature-sensitive mutant (tsA209) of SV40
BioSafety Level:
II
Organism:
Rat (R. norvegicus)
Species:
Rat
Source Organ:
Ovary
Organ Type:
Ovary
Growth Properties:
Adherent/Suspension
Morphology:
Elongated, overlapped and binucleated at 33°C or is mononucleated at 39°C.
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
N/A
Markers:
PCNA
Donor Age:
21 days old
Donor Gender:
Female
Donor Ethnicity:
Not disclosed
Propagation:
The base medium for this cell line is Prigrow II medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) Western blot; 2) Northern blot; 3) Immunofluorescence
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C