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Immortalized Rat Ovarian Granulosa Cells (RGA-1) | T0758

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SKU:
T0758
Availability:
5 to 7 Days Shipment
€4,173.00
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Description

abm | Immortalized Rat Ovarian Granulosa Cells (RGA-1) | T0758

Immortalization Method:

Immortalized with a temperature-sensitive mutant (tsA209) of SV40

BioSafety Level:

II

Organism:

Rat (R. norvegicus)

Species:

Rat

Source Organ:

Ovary

Organ Type:

Ovary

Growth Properties:

Adherent/Suspension

Morphology:

Elongated, overlapped and binucleated at 33°C or is mononucleated at 39°C.

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

PCNA

Donor Age:

21 days old

Donor Gender:

Female

Donor Ethnicity:

Not disclosed

Propagation:

Use of PriCoatTM T25 Flasks or Applied Cell Extracellular Matrix is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow II medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Western blot; 2) Northern blot; 3) Immunofluorescence

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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Additional Information

Size:
1x10⁶ cells / 1.0 ml
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