IP6K1<sup>---<-sup> & IP6K2<sup>---<-sup> Stable HCT116 Cell Line | T6351
- 5 to 7 Days Shipment
abm | IP6K1---<-sup> & IP6K2---<-sup> Stable HCT116 Cell Line | T6351
To study the role that PP-IPs play in mammalian phosphate homeostasis, researchers generated cells devoid of this small molecule. CRISPR was used to create a cell line without PP-IPs. IP6K3 is expressed mainly in muscle cells. Hence researchers chose human colon carcinoma cell line HCT116 as it is near-diploid and expresses IP6K1 and IP6K2 only, and has easily detectable levels of IP7 and IP8. Researchers targeted IP6K1 and IP6K2 using guide RNAs against exon 5 of both genes to disrupt the inositol binding motifs. No IP6K1 or IP6K2 protein as well as IP7/IP8 was detectable in double knockout (DKO) IP6K1/IP6K2/ cell lines.
Human (H. sapiens)
20 - 30 hours
10,000 - 20,000 cells/cm2
IP6K1 and IP6K2 knockout
For Research Use Only
The base medium for this cell line is Prigrow III medium available at abm
1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
1) PCR; 2) Sequencing
10⁶ cells/1.0 ml