IP6K1<sup>---<-sup> Stable HCT116 Cell Line | T6349
- 5 to 7 Days Shipment
abm | IP6K1---<-sup> Stable HCT116 Cell Line | T6349
To study the role that PP-IPs play in mammalian phosphate homeostasis, researchers generated cells devoid of this small molecule. CRISPR was used to create a cell line without PP-IPs. IP6K1-/- line did not have significantly less IP7 than WT (wildtype). IP6K3 is expressed mainly in muscle cells, and the cell lines tested were not muscle-derived and contained no or a very low amount of IP6K3 mRNA. Hence they chose human colon carcinoma cell line HCT116 as it is near-diploid and expresses IP6K1 and IP6K2 only, and has easily detectable levels of IP7 and IP8.
Human (H. sapiens)
58 - 68 hours
30,000 - 40,000 cells/cm2
For Research Use Only
The base medium for this cell line is Prigrow III medium available at abm
1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
1) PCR; 2) Sequencing
10⁶ cells/1.0 ml