Klenow Fragment (3’→5’ Exo-) | E038

abm | Klenow Fragment (3’→5’ Exo-) | E038

Klenow Fragment (3’→5’ Exo-) is the large fragment of E. coli DNA Polymerase I which retains polymerase activity but lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity.

Concentration:

5 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Some Combinations of Overlapping

Application:

• Optimizes cloning efficiency • Reduces false-positive colonies when cloning

Components:

N/A

Inactivation:

N/A

Source:

N/A

Shipping conditions:

Ice Packs

Storage conditions:

Store all components at -20°C.

Storag Buffer:

25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.