Klenow Fragment (3’→5’ Exo-) | E038
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abm | Klenow Fragment (3’→5’ Exo-) | E038
Klenow Fragment (3’→5’ Exo-) is the large fragment of E. coli DNA Polymerase I which retains polymerase activity but lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity.
Enzyme supplied with 10X Reaction Buffer
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Some Combinations of Overlapping
• Optimizes cloning efficiency • Reduces false-positive colonies when cloning
Store all components at -20°C.
25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
500 U (100 μl)