DNA Polymerase I Large (Klenow) Fragment | E013
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abm | DNA Polymerase I Large (Klenow) Fragment | E013
DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.
Enzyme supplied with 10X Reaction Buffer
One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.
This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA.
This product is distributed for laboratory research only
Caution: Not for diagnostic use.
Store all components at -20°C.
25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.