DNA Polymerase I Large (Klenow) Fragment | E013

abm | DNA Polymerase I Large (Klenow) Fragment | E013

DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.

Concentration:

5 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.

This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA.

This product is distributed for laboratory research only
Caution: Not for diagnostic use.

Application:

N/A

Components:

N/A

Inactivation:

N/A

Source:

E. coli

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.