LN229 Stably Expressing Tet-on Cell Line | T6030

abm | LN229 Stably Expressing Tet-on Cell Line | T6030

LN229 cells were transfected with the rtTA expression vector to generate the LN229 Stably Expressing Tet-on Cell Line.LN229 have heterogeneous backgrounds for PTEN, mutated p53, and deletion of p16 and p14ARF. It can be used in vitro to stably transfect gene of interest cloned in tetracycline-responsive expression vector (i.e. pTRE2 with Hygromycin selection) and pick clones. With the addition of 1 µg/ml doxycycline, end users can test individually for induced gene expression under the tet-regulated promoter.

Biosafety:

II

Organism:

Human

Source Organ:

Brain

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfection with rtTA expression vector

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

Selection with 0.25 ug/ml puromycin (G264)

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C