abm drug discovery
NF-κB Reporter & CD14 Stable Expressing CHO Cell Line (3E10) | T6435
- SKU:
- T6435
- Availability:
- 5 to 7 Days Shipment
Description
abm | NF-κB Reporter & CD14 Stable Expressing CHO Cell Line (3E10) | T6435
The NF-κB Reporter & CD14 Stable Expressing CHO Cell Line (3E10) was generated by stably transfecting CHO cells with a reporter construct containing the gene for CD25, regulated by the human E-selectin promoter. The human E-selectin promoter contains a NF-κB binding site making the expression of CD25 dependent on the translocation of NF-κB to the nucleus.
Biosafety:
II
Organism:
Chinese Hamster (C. griseus)
Source Organ:
Ovary
Growth Properties:
Adherent
Morphology:
Epithelial-Like
Clones:
N/A
Passage Number:
N/A
Population Doner:
24 - 34 hours
Seeding Density:
20,000 - 40,000 cells/cm2
Markers:
Inducible membrane CD25 under the control of nuclear factor-κB (NF-κB) Hygromycin B and Ciprofloxacin Resistant
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
Flow cytometry using a FACScan microfluorometer & Western Blot
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C