TLR4 & CD14 Stable Expressing CHO Cell Line (3E10 TLR4) | T6436

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SKU:
T6436
Availability:
5 to 7 Days Shipment
€4,173.00
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Description

abm | TLR4 & CD14 Stable Expressing CHO Cell Line (3E10 TLR4) | T6436

Toll-like receptor 4 is a signal transducer that is known to implicate cellular responses to lipopolysaccharides. To generate the TLR4 & CD14 Stable Expressing CHO Cell Line (3E10 TLR4) CHO/CD14 reporter cells were stably transfected with the human cDNA for TLR4 in the pFLAG-CMV-1 vector.

Biosafety:

II

Organism:

Chinese Hamster (C. griseus)

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Epithelial-Like

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 30 hours

Seeding Density:

20,000 - 40,000 cells/cm2

Markers:

Inducible membrane CD25 under the control of nuclear factor-κB (NF-κB) and Toll-like Receptor 4 Hygromycin B, Ciprofloxacin, and Puromycin Resistant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoatTM T25 Flasks

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

Flow cytometry using a FACScan microfluorometer & Western Blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C

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Additional Information

Size:
10⁶ cells/1.0 ml
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