NonO Stable Knockdown Jurkat Cell Line | T6124
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abm | NonO Stable Knockdown Jurkat Cell Line | T6124
NonO (Non-POU domain-containing octamer-binding) protein is called p54nrb (nuclear RNA binbing) is encoded by the NONO gene. It has been associated with melanoma, and has strongh expression in melanoma tissue samples. NonO-specific shRNA-expressing GFP lentiviral vector pGIPZ was used to knockdown NonO expression in Jurkat cells to generate the NonO Stable Knockdown Jurkat Cell Line to study the effects and role of NonO and cancer.
For Research Use Only
The base medium for this cell line is Prigrow II medium available at abm
1. Freeze Medium: Complete growth medium with 20% FBS and 5% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
1) To determine knockdown of NonO, anti-NonO antibody (abcam, ab13164) was used for immunoblotting.
10⁶ cells/1.0 ml